A Guide to Zona Pellucida Domain Proteins by Eveline S. Litscher, Paul M. Wassarman

By Eveline S. Litscher, Paul M. Wassarman

This ebook presents a coherent, transparent, and uniform presentation of structural, genetic, molecular, and biochemical details on hand for the zona pellucida area protein relations, which influence pathologies corresponding to infertility, deafness, and melanoma. moreover it:

  • Details information regarding the constitution and serve as of the ZP area in ZPDC-proteins
  • Provides illustrations of the association of ZPDC-proteins, the genes that encode the proteins, and examples of mutations within the ZP area that reason diseases
  • Speculates as to the evolution of the ZP area and strength therapeutics for illnesses stemming from ZP area mutations
  • Addresses mammalian and non-mammalian systems

Show description

Read Online or Download A Guide to Zona Pellucida Domain Proteins PDF

Best molecular biology books

Basic Mathematics for Biochemists

A few academics of biochemistry imagine it certainly valuable for college students to fight with tricky arithmetic. i don't quantity myself between those humans, even if i've got derived a lot own excitement from the research of arithmetic and from using it to difficulties that curiosity me in biochemistry. to the contrary, i believe that scholars decide upon classes in biochemistry out of curiosity in biochemistry and they shouldn't be weighted down with extra arithmetic than is basically required for a formal figuring out of biochemistry.

Targeted Therapy of Acute Myeloid Leukemia

This publication presents an remarkable assessment of "Targeted remedies" for acute myeloid leukemias. It goals at a virtually accomplished insurance of the various healing thoughts which have been built over the past decade and are actually being evaluated in early scientific trials. Paired and authoritative chapters via top study scientists and clinicians clarify uncomplicated innovations and scientific translation of subject matters that come with the underlying genetic and proteomic abnormalities of AML, the advance of novel nucleoside analogues, the jobs of microRNAs, apoptosis regulators Bcl-2 and p53 and of severe cellphone signaling proteins akin to PIM, FLT3, Raf/MEK, PI3K/AKT/mTOR and aurora kinases.

Ras Signaling Methods and Protocols

That includes experimental techniques that make clear the complexity of Ras GTPase organic functions, Ras Signaling equipment and Protocols contains basic overviews and specified purposes of either well-established and lately constructed learn strategies, together with biochemical, biophysical, molecular biology, genetic and behavioral techniques, complex excessive solution fluorescence and electron microscopy imaging and “omics” applied sciences.

Extra resources for A Guide to Zona Pellucida Domain Proteins

Example text

1 mtggrlvall llvaaslglg qqphpepglp glqysydcgm rgmqlvvlpr pgrtirfkvv 61 defgnrfevn ncsicfhwvs aepqapavfs adykgchvle keghshltvf ieamlpdghv 121 evaqeavlic pkpghtwavg shqvppttps pttphalpfh lssahtfpip lylehslmlp 181 tpagpslgpg ptpavlaqve rwevdkpdav gshlpqewCq vasghipCiv qssskeaCeq PLACENTAL MAMMALS 31 241 agCCydsare vpCyygntat vqCfrngyfi lvvaqemala hritlanvhl ayaptrCppa 301 qktsafvifh vplthCgttv qvlgsqlfye nqlvsdidvr egpqgsitrd ssfrllvrCi 361 fnasdflpiq asifspplpa pvtqagplrl elriardetf ssfyeeedyp lvrllrepvh 421 vevrllqrtd pslvlelhqC watpsanpvq qpqwpllsdg Cpfkgdsyrt rvlaldrael 481 pfrshyqrft vatftfldsg aqralrglvy ffCsasaChp sgpetCsstC ssrtakrrrs 541 sgyhdgtpra ldivsspgpv gfqdshrqep tlestgsgrn snpkpllwvv llllaialvl 601 gigvfvglsq awahklregh rltdqaq ZP2: The polypeptide of ZP2 consists of 727 aa residues and has a ZPD (aa 380–644; highlighted) with 10 Cys residues (aa 381, 412, 433, 474, 554, 575, 624, 629, 639, 643; capitalized and underlined), followed by a CFCS (aa ­648–651, RHRR; highlighted and underlined) and TMD (aa 698–717 highlighted).

Mouse ZP genes share TATAA boxes ≃30 bp upstream of the transcription start sites and E‐box sequences (CANNTG) that are involved in oocyte‐specific expression of ZP genes. At least two ovary‐specific DNA‐binding proteins, ZAP‐1 and OSP‐1, bind to promoters of mZP2 and 3. , firefly luciferase) to growing oocytes. Messenger‐ RNA encoding mouse ZP proteins is undetectable in non‐growing oocytes but appears in small oocytes that have entered the growth phase. For example, messenger‐RNA encoding mZP3 is undetectable in non‐growing oocytes but increases to ≃300,000 copies/oocyte in mid‐stage growing oocytes, falls to ≃240,000 copies/oocyte in fully‐grown oocytes, and decreases to undetectable levels in fertilized eggs (<1000 copies/egg).

Inhibition of zona pellucida gene expression by antisense oligonucleotides injected into mouse oocytes. J Biol Chem 270, 849–853 (1995). Wassarman PM. Fertilization in the mouse. I. The egg. ), Plenum Press, New York, pp. 151–165 (1991). Wassarman PM, Litscher ES. Influence of the zona pellucida of the mouse egg on folliculogenesis and fertility. Int J Dev Biol 56, 833–839 (2012). Wassarman PM, Litscher ES. Biogenesis of the mouse egg’s extracellular coat, the zona ­pellucida. Curr Topics Dev Biol 102, 243–266 (2013).

Download PDF sample

Rated 4.45 of 5 – based on 31 votes